Coding

Part:BBa_K810000:Design

Designed by: Spencer R Katz   Group: iGEM12_Yale   (2012-09-28)


Lambda phage beta CDS with T5 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 636
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 636
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 636
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CDS for beta is downstream of the T5-lac promoter from plasmid pBAV1K-T5 described by Bryksin and Matsumura, 2010. The construct was designed to be cloned into pBAV1K to create a construct that could be used across species.


Source

The promoter region was amplified from the plasmid pBAV1K-T5-gfp (Bryksin and Matsumura, 2010). The coding sequence was amplified from E. coli strain SIMD50 as described in Datta, et al., 2006. This coding sequence is originally from the bacteriophage lambda.

References